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技术研究项目情况
阅读次数:738   发布时间: 2015/11/2 16:11:27   文章来源:  作者:admin    

项目情况

p  国家现代农业产业技术体系——国家绒毛用羊产业技术体系岗位科学家;

p  国家现代农业产业技术体系——国家肉羊产业技术体系昆明综合试验站建设;

p  科技部家养动物平台

p  国家公益性行业(农业)科研专项——奶山羊良种繁育及产业化技术体系建设;

p  中央补助地方科技基础条件专项资金项目——云南绵羊、山羊种质资源保存中心建设;

p  国家自然科学基金——肌醇类SIB维持绵羊冷冻精子细胞结构和遗传信息稳定性的机制;

p  国家自然科学基金——结合蛋白质组学和RNA-Seq技术分析海藻糖对绵羊冷冻精子的保护机制;

p  国家自然科学基金——GV期卵母细胞冷冻保存的内质网应激损伤及干预研究;

p  2014年云南省高新技术产业发展项目——云南省主要家畜遗传资源保存与选育关键技术研究与应用;

p  云南省应用基础面上项目——玻璃化冷冻保存猪GV期卵母细胞的内质网损伤机制研究;

p 云南省应用基础面上项目——通过体外拟合基因群mRNA转录模式研究羊毛相关基因功能。

发表论文

1)   Guo Bo Quan, Dong Jiang Li, Yuan Ma, Lan Zhu, Chun Rong Lv, Qiong Hua Hong. Cryopreservation of ram spermatozoa in the presence of cyclohexanhexol-derived synthetic ice blocker. Small Ruminant Research, 123:110-117, 2015

2)   Guo Bo Quan, Yuan Ma, Jian Li, Guo Quan Wu, Dong Jiang Li, Yi Na Ni, Chun Rong Lv, Lan Zhu, Qiong Hua Hong. Effects of Hoechst33342 staining on the viability and flow cytometric sex-sorting of frozen-thawed ram sperm. Cryobiology, 70:23–31, 2015

3)   Wu G, Jia B, Mo X, Liu C, Fu X, Zhu S, Hou Y. Nuclear maturation and embryo development of porcine oocytes vitrified by cryotop: effect of different stages of in vitro maturation. Cryobiology201367: 95-101.

4)   Wu GQ, Jia BY, Li JJ, Fu XW, Zhou GB, Hou YP, Zhu SE. L-carnitine enhances oocyte maturation and development of parthenogenetic embryos in pigs. Theriogenology201176785-793.

5)   Jia B, Wu G, Fu X, Mo X, Du M, Hou Y, Zhu S. trans-10, cis-12 conjugated linoleic acid enhances in vitro maturation of porcine oocytes. Mol Reprod Dev., 201481: 20-30.

6)   Fu XW, Wu GQ, Li JJ, Hou YP, Zhou GB, Lun-Suo, Wang YP, Zhu SE. Positive effects of Forskolin (stimulator of lipolysis) treatment on cryosurvival of in vitro matured porcine oocytes. Theriogenology201175: 268-275.(同等贡献)

7)         Li JJ, Pei Y, Zhou GB, Suo L, Wang YP, Wu GQ, Fu XW, Hou YP, Zhu SE. Histone deacetyltransferase1 expression in mouse oocyte and their in vitro-fertilized embryo: effect of oocyte vitrification. CryoLetters 201132: 13-20.

8)   Guo Bo Quan, Wei Juan Li, Zhi Gang Lan, Shuai Shuai Wu, Qing Yong Shao, Qiong Hua Hong. The effects of meiotic stage on viability and developmental capability of goat oocytes vitrified by the Cryoloop method. Small Ruminant Research116 32– 362014.

9)   Chun-Lei ChenXia-Fei FuLin-Qing WangJun-Jie WangHua-Gang MaShun-Feng ChengZhu-Mei HouJin-Mei MaGuo-Bo QuanWei ShenLan Li. Primordial follicle assembly was regulated by notch signaling pathway in the mice. 41(3): 1891-18992014.

10) Quan GB, Wu SS, Lan ZG, Yang HY, Shao QY, Hong QH. The effects of 1,4-cyclohexanediol on frozen ram spermatozoa. CryoLetters34 (3) 217-2272013.

11) Wu SS, Li DJ, Lv CR, Yang HY, Zhu L, Li WJ, Quan GB, Hong QH. Establishment and cryopreservation of a skin fibroblast cell line derived from Yunnan semi-fine wool sheep in the presence of synthetic ice blocker. CryoLetters34 (5)497-5072013.

12) Quan GB, Hong QH, Shao QY, Yang HY, Wu SS. The effects of trehalose and sucrose on frozen spermatozoa of Yunnan semi-fine wool sheep during a non-mating season. CryoLetters33 (4)307-317, 2012.

13) Quan GB, Hong QH, Lan ZG, Yang HY, Wu SS. Comparison of the Effect of Various Disaccharides on Frozen Goat Spermatozoa. Biopreservation and Biobanking, 105):439-445, 2012.

14) Quan GB, Han Y, Liu MX, Fang L, Du W, Ren SP, Wang JX, Wang Y. Addition of oligosaccharide decreases the freezing lesions on human red blood cell membrane in the presence of dextran and glucose. Cryobiology62135-144, 2011.

15) Wang JX, Yang C, Wang Y, Liu MX, Ren SP, Quan GB, Han Y. Stability of lyophilized human platelets loaded with small molecule carbohydrates. CryoLetters32 (2)123-130, 2011.

16) Ren SP, Howell PM, Han Y, Wang JX, Liu MX, Wang Y, Quan GB, Du W, Fang L, Riker AI. Overexpression of the Progestagen-associated endometrial protein gene is associated with microphthalmia-associated transcription factor in human melanoma. The Ochsner Journal, 11: 212-219, 2011.

17) Quan GB, Liu MX, Han Y, Fang L, Du W, Wang JX. In vivo circulation of mouse red blood cells frozen in the presence of dextran and glucose. Cryobiology61(1): 10-162010.

18) Quan GB, Han Y, Liu MX, Gao F. Effects of pre-freeze incubation of human red blood cells with various sugars on postthaw recovery when using a dextran-rapid cooling protocol. Cryobiology59258-267, 2009.

19) 权国波,杨红远,李东江,李卫娟,洪琼花。寡聚糖对云岭黑山羊精液的冷冻保护效果分析。黑龙江动物繁殖,214):7-112013

20) 权国波,李东江,黄羽,李卫娟,洪琼花。肌醇类SIB对云南半细毛羊精液冷冻保护效果影响的研究。中国草食动物科学,334):14-182013

21) 兰志刚,李东江,李卫娟,吕春荣,杨红远,洪琼花。山羊卵母细胞体外培养体系及冻存后体外培养的研究。中国草食动物科学,224-27, 2012

22) 兰蓉,朱兰,王鹏,洪琼花,胡钟仁。云南肉山羊黑色品系内不同家系遗传多样性分析。中国草食动物科学,28-12, 2012

23) 杨红远,洪琼花,邵庆勇,汤守琨。季节对沼泽型水牛采卵效果和卵母细胞体外成熟的影响。中国草食动物科学,227-292012

24) 邵庆勇,杨红远,牛自兵,李卫娟,朱兰,吕春荣,洪琼花。水牛卵母细胞冷冻保存初步研究。中国草食动物,612-162011

25) 杨红远, 洪琼花, 赵智勇, 赵家明。成熟时间对水牛卵母细胞体外成熟的影响研究。家畜生态学报,311):83-852010

26) 邵庆勇,于钦秀,赵智勇,洪琼花。不同发育阶段圭山山羊胚胎常规冷冻保存研究。中国草食动物,306):15-172010

申请专利

1)       洪琼花,权国波,杨红远,李东江,吕春荣,一种家畜冷冻稀释液及其制备方法和应用,2012.10,中国,专利号:ZL 201210491289.2

2)       邵庆勇,李卫娟,权国波,李东江,黄羽,洪荣,一种羔羊保育箱,2013.12,中国,专利号:ZL 201320360194.7

3)       邵庆勇,洪琼花,权国波,李卫娟,李东江,黄羽,洪荣,一种羔羊补饲槽,2013.12,中国,专利号:ZL 201320360246.0

4)       权国波;韩 颖;刘敏霞;郭 永;张 亮;王 艳;王捷熙;刘 安,红细胞深低温保存预处理液、冷冻保护液及其应用,2008.06,中国,专利号:ZL200610066310.9

5)       王捷熙,韩 颖,杨 超,王 艳,刘敏霞,权国波,刘 安,一种用于血小板冰冻干燥保存的小分子糖稳定缓冲液,2010.06,中国,专利号:ZL200710176773.5

6)                   王 艳,杨 睿,韩 颖,王捷熙,刘敏霞,权国波,刘 安,一种基于十四烷的定形相变材料及其制造方法,2011.06,中国,专利号:ZL200710176686.X


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